Mouse model: C57BL/6NJ-Polg^em1Murr/Mmjax

Polg-floxed mice possess loxP sites flanking exon 3 of the Polg gene. These mice may be useful for generating conditional mutants when studying mitochondrial genome replication and mitochondrial associated disorders. Polg codes for polymerase (DNA directed), gamma, which is the catalytic subunit of mitochondrial DNA polymerase and necessary for replication of mitochondrial DNA. Mutations in this gene can lead to mitochondrial disorders that may present progressive myopathy, developmental delay, epilepsy, ataxia and ophthalmoplegia. These Polg-floxed mice harbor a CRISPR/Cas9-generated floxed allele, with loxP sites flanking exon 3 of the Polg gene.Mice homozygous for this floxed allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted resulting in a frameshift and early truncation at amino acid position 213. The donating laboratory bred these Polg-floxed mice to a Sox2-Cre mouse line to generate global Polg knock-out (KO) mice. The resulting Polg KO mice were found to be embryonic lethal. Polg-floxed mice may be useful for generating conditional mutants for studying mitochondrial DNA replication and mitochondrial dysfunction.

The mouse model C57BL/6NJ-Polg^em1Murr/Mmjax is now available:

https://www.jax.org/strain/037441

For more information please do not hesitate to contact research@polgfoundation.org